Alternative conceptions are widespread, resistant to change, and hinder effective learning. The objective of this research project is to analyze, through a systematic review of the literature, the characteristics, theoretical framework, and effectiveness of instructional interventions aimed at overcoming alternative conceptions in chemistry among secondary school and university students. In November 2024, a systematic review was conducted across 24 databases on the ProQuest portal. The search returned a total of 1,618 articles, which were assessed to determine their compliance with the eligibility criteria. A total of 84 intervention studies were included in the systematic review. The analyzed studies are diverse in terms of duration, included populations, covered topics, theoretical backgrounds, teaching methodologies, and assessment methods. All the interventions demonstrated that the use of an active teaching methodology is more effective than traditional lectures in promoting conceptual change. The adoption of active teaching methods can enhance the learning of chemistry. Future studies could investigate which type of active learning is most effective for specific topics or student groups.